Figure 5. MMP-12 cleaves recombinant PGRN.

(A) Varying concentrations of recombinant PGRN (0.05 or 0.1 µM) were incubated with or without activated recombinant MMP-12 (0.1 µM) in a specified assay buffer at 37°C for 40 min or 18 h. Samples were fractionated using a 4–15% gradient gel. (B) MMP-12 dose response: PGRN was incubated with increasing concentrations (0.01–0.3 µM) of MMP-12 for 2 h, then separated using a 4–15% gradient gel. In both samples, five different PGRN cleavage products were noted corresponding to ∼45 kDa, 35 kDa, 25 kDa, 19 kDa and 12 kDa (arrowheads).