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. 2012 Apr 11;7(4):e35115. doi: 10.1371/journal.pone.0035115

Figure 8. Role of microglial PGRN in TLR3/4-mediated cytokine production.

Figure 8

Microglial cultures were treated with siRNA specific for human PGRN (PGRN-si: white symbol) or a control non-targeting siRNA (cont-si: black symbol) for 3 days. Cultures were then stimulated with LPS or poly IC for additional 24 h. (A) PGRN ELISA was performed to determine the effect of PGRN-si in microglia. (B to H) TNFα, IL-6, IL-1β, IL-1ra, IL-10, IP-10 and IL-8 were measured by ELISA in the same culture. Results from a representative experiment are shown. Data are mean ± SD from triplicate samples (* p<0.05, ** p<0.01, ***p<0.001). The results show that PGRN-si reduces the production of multiple cytokines and chemokines induced by LPS or poly IC.