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. 2012 Jan 31;33(4):760–769. doi: 10.1093/carcin/bgs023

Fig. 7.

Fig. 7.

Knockdown of ETS2 or overexpression of miR-196b resulted in differential expression of EMT-related genes in gastric cancer cells. (A) HR cells expressed high levels of vimentin, MMP2 and MMP9, but AGS cells expressed high levels of E-cadherin. The expression patterns of genes were verified by RT–qPCR. (B) The gene expression patterns of E-cadherin, vimentin, MMP2 and MMP9 were detected by RT–qPCR in shETS2-treated AGS cells and ETS2-overexpressing HR cells. AGS cells expressed low levels of vimentin; increased expression of vimentin was detected after ETS2 knockdown in AGS cells (western blot). Antibodies to vimentin and actin (internal controls) were used. (C) The gene expression patterns of E-cadherin, vimentin, MMP2 and MMP9 were detected by RT–qPCR in miR-196b-treated AGS cells and antagomir-196b-treated HR cells. (D) The gene expression patterns of E-cadherin, MMP2 and MMP9 were detected by RT–qPCR in antagomir-196b-treated AGS–shETS2 cells. The expression levels of EMT-related genes were detected using RT–qPCR by the SYBR Green I protocol. All values were normalized against glyceraldehyde 3-phosphate dehydrogenase mRNA. shLuc and mock were used as negative controls. The data are an average of triplicates for each condition. *P < 0.05, **P < 0.01.