Fig. 6.

Bortezomib skews the phenotype of RAW264.7 mouse macrophages in vitro. Wild-type and NF-κΒ reporter (pNGL) RAW264.7 cells were cultured with varying concentrations of bortezomib. (A) Cellular proliferation of wild-type cells at 48 h. (B) NF-κΒ-dependent luciferase activity of pNGL cells at 4 h. (C) Inflammatory mediator expression at 48 h. All experiments were done thrice; n = 3, independent observations for all experiments. IL, interleukin; TNF, tumor necrosis factor; IFN, interferon; n, sample size; P, probability. Columns, mean; bars, SD. *, ** and ***: P < 0.05, 0.01 and 0.001, respectively, compared with bortezomib 0 ng/ml (no treatment).