Fig. 5.

Overexpression of PP2A or PP5 partially prevents curcumin-induced activation of JNK and Erk1/2, as well as cell death. Rh30 cells, infected with Ad-PP2A, Ad-PP5 or Ad-LacZ (as control), were exposed with curcumin (0–20 μM) for 4 h (for western blotting) or 72 h (for apoptosis assay), followed by western blot analysis using indicated antibodies (A and B) or apoptosis assay using Annexin V-FITC Apoptosis Detection Kit I (C). Results (C) are presented as mean ± SE (n = 3). ^aP < 0.05, difference versus control group; ^bP < 0.05, difference with 10 μM curcumin group with Ad-LacZ infection; ^cP < 0.05, difference with 20 μM curcumin group with Ad-LacZ infection.