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. 2012 Apr 11;7(4):e33607. doi: 10.1371/journal.pone.0033607

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Size exclusion elution profiles from a Superdex 200 column are displayed. A, Concentrated protein applied directly to the column, with no pre-incubation period, elutes as three species with sizes consistent with monomeric, dimeric and tetrameric forms of the protein. B, Following an incubation period of two hours, the protein elutes as a homogeneous tetrameric species.