Table 1. Sequenced urinary biomarkers for drug induced kidney injury.
| Mass [Da] | CE-T [Min] | Regulation factor in gentamicin study | p-value (BH) | AUC | Sequence | UniProt name (start AA- stop AA) |
| 1197.58 | 36.97 | 12.5 | 2.9E−04 | 0.793 | DSYVGDEAQSK | ACTS_RAT (53–63) |
| 1685.83 | 41.55 | 4.7 | 2.1E−02 | 0.688 | AFSPVASVESASGEVLH | FETUA_RAT (307–323) |
| 1215.56 | 38.53 | 1234.6 | 1.3E−03 | 0.688 | TIDQNLEDLR | APOA4_RAT (211–220) |
| 2070.03 | 30.75 | 9.8 | 2.5E−04 | 0.823 | AEGSpGRDGApGAKGDRGETGP | CO1A1_RAT (1009–1030) |
| 1998.98 | 30.79 | 86.1 | 0.0E+00 | 0.998 | EGSpGRDGApGAKGDRGETGP | CO1A1_RAT (1010–1030) |
| 1366.66 | 39.46 | 16.1 | 1.7E−02 | 0.667 | DRGETGPAGPpGApG | CO1A1_RAT (1024–1038) |
| 2047.06 | 36.4 | 9.3 | 6.9E−04 | 0.798 | GApGApGPVGPAGKNGDRGETGP | CO1A1_RAT (1038–1060) |
| 1990.04 | 35.94 | 5.0 | 2.4E−05 | 0.872 | ApGApGPVGPAGKNGDRGETGP | CO1A1_RAT (1039–1060) |
| 1805.94 | 34.27 | 13.9 | 0.0E+00 | 0.959 | GApGPVGPAGKNGDRGETGP | CO1A1_RAT (1041–1060) |
| 2048.01 | 30.91 | 87.4 | 1.8E−03 | 0.710 | NGDDGEAGKPGRPGERGppGP | CO1A1_RAT (218–238) |
| 1876.95 | 31.11 | 109.9 | 6.4E−04 | 0.793 | DDGEAGKPGRPGERGPpGp | CO1A1_RAT (220–238) |
| 1646.89 | 27.78 | 10.2 | 1.5E−02 | 0.724 | GEAGKPGRpGERGPpGP | CO1A1_RAT (222–238) |
| 1662.89 | 27.84 | 7.3 | 3.8E−03 | 0.764 | GEAGKpGRpGERGPpGP | CO1A1_RAT (222–238) |
| 2040.99 | 36.17 | 100.9 | 0.0E+00 | 0.973 | NSGEpGApGNKGDTGAKGEpGP | CO1A1_RAT (421–442) |
| 1737.87 | 41.58 | 5.8 | 3.3E−02 | 0.700 | TGSpGSpGPDGKTGPpGPAG | CO1A1_RAT (530–549) |
| 1235.62 | 38.24 | 9.2 | 2.0E−03 | 0.776 | pGPDGKTGPpGPAG | CO1A1_RAT (536–549) |
| 1691.86 | 35.21 | 5.1 | 1.0E−05 | 0.898 | DGKTGPpGPAGQDGRPGp | CO1A1_RAT (539–556) |
| 1737.9 | 33.68 | 75.2 | 6.9E−04 | 0.720 | GTAGEpGKAGERGVpGPpG | CO1A1_RAT (576–594) |
| 1508.78 | 32.47 | 146.3 | 5.9E−05 | 0.785 | GEpGKAGERGVpGPpG | CO1A1_RAT (579–594) |
| 1451.77 | 32.23 | 6.0 | 8.2E−03 | 0.831 | EpGKAGERGVpGPpG | CO1A1_RAT (580–594) |
| 1550.78 | 40.72 | 76.6 | 4.8E−04 | 0.726 | VGPAGKDGEAGAQGApGP | CO1A1_RAT (596–613) |
| 1405.72 | 39.05 | 3.4 | 1.8E−04 | 0.827 | GLpGPAGPpGEAGKpG | CO1A1_RAT (633–648) |
| 1860.95 | 42.67 | 4.9 | 3.7E−04 | 0.777 | TGPIGPpGPAGApGDKGETGP | CO1A1_RAT (755–775) |
| 1584.8 | 33.97 | 19.6 | 6.0E−06 | 0.904 | DGQPGAKGEpGDTGVKG | CO1A1_RAT (809–825) |
| 1469.76 | 32.4 | 6.0 | 3.4E−03 | 0.766 | GQPGAKGEpGDTGVKG | CO1A1_RAT (810–825) |
| 1182.6 | 39.65 | 4.0 | 4.0E−05 | 0.873 | DTGVKGDAGPpGP | CO1A1_RAT (820–832) |
| 1067.56 | 36.83 | 9.4 | 1.5E−02 | 0.724 | TGVKGDAGPpGP | CO1A1_RAT (821–832) |
| 1844.97 | 34.95 | 18.4 | 2.0E−06 | 0.927 | SGNAGPpGPpGPVGKEGGKGP | CO1A1_RAT (878–898) |
| 2058.09 | 30.81 | 108.6 | 2.4E−04 | 0.758 | SGNAGPpGPpGPVGKEGGKGPRG | CO1A1_RAT (878–900) |
| 1590.87 | 27.67 | 5.8 | 3.0E−06 | 0.920 | GPpGPVGKEGGKGPRGE | CO1A1_RAT (885–901) |
| 1900.95 | 42.53 | 7.0 | 4.5E−02 | 0.655 | ETGPAGRpGEVGPpGPpGPAG | CO1A1_RAT (901–921) |
| 1714.81 | 42.32 | 119.1 | 2.4E−02 | 0.641 | TGPAGRpGEVGPpGPpGPA | CO1A1_RAT (902–920) |
| 1771.91 | 41.88 | 13.0 | 2.9E−03 | 0.756 | TGPAGRpGEVGPpGPpGPAG | CO1A1_RAT (902–921) |
| 1388.72 | 39.15 | 2.2 | 1.5E−05 | 0.866 | RpGEVGPpGPpGPAG | CO1A1_RAT (907–921) |
| 1944.02 | 35.43 | 13.6 | 3.0E−06 | 0.908 | RpGEVGPpGPpGPAGEKGSPG | CO1A1_RAT (907–927) |
| 1308.64 | 39.09 | 4.7 | 1.0E−02 | 0.707 | GLpGPSGEPGKQGp | CO1A1_RAT (963–976) |
| 1314.6 | 48.73 | 36.7 | 4.1E−02 | 0.624 | GNpGPpGPpGPpGPG | CO2A1_RAT (1135–1149) |
| 1441.74 | 32.22 | 31.9 | 0.0E+00 | 0.946 | SpGIpGPKGEDGKDG | CO3A1_RAT (453–467) |
| 1695.81 | 40.95 | 32.4 | 4.8E−03 | 0.720 | GMpGSpGGPGNDGKpGPpG | CO3A1_RAT (536–554) |
| 1351.66 | 39.57 | 6.1 | 6.0E−06 | 0.904 | ApGDKGDAGPpGPQG | CO3A1_RAT (624–638) |
| 1558.73 | 48.82 | 10.0 | 7.0E−06 | 0.892 | GLpGPpGNNGNpGPpGP | CO3A1_RAT (878–894) |
| 2071.06 | 36.16 | 4.9 | 4.0E−02 | 0.678 | VGEpGPAGSKGETGNKGEpGSAG | CO1A2_RAT (351–373) |
| 1620.84 | 40.7 | 55.9 | 2.5E−04 | 0.757 | GLpGSpGNVGPAGKEGPV | CO1A2_RAT (457–474) |
| 1677.84 | 41.02 | 46.6 | 3.3E−02 | 0.749 | GLpGSpGNVGPAGKEGPVG | CO1A2_RAT (457–475) |
| 1142.56 | 36.71 | 10.4 | 3.1E−03 | 0.751 | NIGFpGPKGPSG | CO1A2_RAT (497–508) |
| 1414.68 | 39.01 | 4.9 | 5.9E−03 | 0.753 | LYQAEAFIADFK | SPA3L_RAT (156–167) |
| 989.49 | 35.54 | 26.6 | 9.3E−03 | 0.690 | IDELYLPK | SPA3N_RAT (306–313) |
| 1690.86 | 41.54 | 13.0 | 2.2E−02 | 0.691 | EPPSLRPAPPPISGGGY | FIBB_RAT (43–59) |
| 1711.8 | 40.76 | 7.5 | 2.0E−02 | 0.759 | DSFGDLSSASAImGNPK | HBB1_RAT (44–60) |
| 1163.56 | 37.95 | 6.7 | 2.8E−02 | 0.720 | LGDGLVGSRQY | O35802_RAT (651–661) |
| 1416.68 | 39.34 | 9.0 | 1.1E−02 | 0.707 | ISHELESSSSEVN | OSTP_RAT (305–317) |
| 1583.81 | 33.26 | 119.5 | 2.8E−02 | 0.740 | DGTDYKTLLSRQMG | EGF_RAT (501–514) |
| 2082.11 | 31.05 | 44.6 | 4.3E−02 | 0.633 | SLTDKTEKELLDSYIDGR | THRB_RAT (342–359) |
| 1486.72 | 39.89 | 13.2 | 2.6E−02 | 0.684 | EELDHALNDMTSI | TPM1_RABIT (272–284) |
Given are molecular mass (in Da), normalized migration time (in min) regulation factor (mean signal intensity of urine samples from 150 and 300 mg/kg gentamicin treated rats collected at days 3, 7 and 10 divided by mean signal intensity of control samples), adjusted p-value (Benjamini and Hochberg), amino acid sequence (modified amino acids: p = hydroxyproline; k = hydroxylysine; m = oxidized methionine) and short protein name of the UniProt database with the position of the firs and last amino acid in parenthesis.