Skip to main content
. 2012 Apr 11;7(4):e34132. doi: 10.1371/journal.pone.0034132

Figure 3. SaliPhe and bafilomycin protect against wear particle-induced osteolysis in vivo– histological and histomorphometric analysis.

Figure 3

(A) Histological H&E appearance of murine calvarial tissues along the middle suture. Both H&E and TRAP staining were taken for at least 3 sections for each group. The sham section shows few inflammatory and osteolytic changes. The vehicle group shows marked inflammatory reaction and prominent osteolysis, whereas the inhibitor treated groups reveal a reduction in both inflammation and osteolysis. White arrowheads indicate TRAP positive osteoclasts. (B–D) Histomorphometric analysis of bone volume against tissue volume (% BV/TV), erosion area (the percentage of infiltration fibrotic area against total tissue area), and the number of TRAP positive multinucleated osteoclasts (>3 nuclei) on the bone surface was measured (*P<0.05, **P<0.01 against vehicle control).