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Coomassie-stained 12% SDS/PAGE (A). Lane 1 is 5 μg Mark12 wide range protein standard (NOVEX, San Diego). The molecular masses of the standard proteins are indicated. Lane 2 is 1 μg purified GroEL of A. vinelandii (molecular weight = 58,800). Western blot with commercially available antibodies raised against E. coli GroEL (B). Lane 1 is 20 μg of crude extract of A. vinelandii strain CA12 (ΔnifHDK), and lane 2 is 1 μg purified GroEL of A. vinelandii.
