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. 2012 Apr 11;7(4):e34711. doi: 10.1371/journal.pone.0034711

Figure 4. Transfection efficiencies and cytotoxicities of chitosan, Chi-g-bPEIx and bPEIs in three cell lines.

Figure 4

Transfection efficiencies (A, C, E) and cytotoxicities (B, D, F) in HeLa (A, B), COS-7 (C, D) and U87-MG (E, F) cell lines. Chitosan, Chi-g-bPEIx copolymers and 2 kDa branched polyethylenimine (bPEI) were used at nitrogen (N) to plasmid DNA phosphate (P) ratio (N/P) 30 and 25 kDa bPEI was administered at N/P 10 according to the existing literature. Results are expressed as mean ± standard deviation (n≥4) (*, p<0.05 vs. Chi-g-bPEI2.7%; #, p<0.05 vs. 2 kDa bPEI).