Figure 4. Effect of co-immunization with Gag-Pol-Nef and gp120 on Gag- and Pol-specific CD8⁺ T cell responses.

BALB/c mice (n = 6 per group) were i.m. inoculated (+) with ^MG^FSPN; or equimolar mixtures of ^MG^FSPN and gp120; ^MGag and PN; or ^MGag, PN, and gp120. After 12 days, spleen cells were isolated and tested for specific cellular immune responses by measuring IFNγ production after stimulation with Gag (black), Pol (grey) or Env (hatched) specific peptides as described in Fig. 3. Cell culture medium served as a negative control. Data shown are representative of two experiments.