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. 2012 Apr 11;7(4):e34723. doi: 10.1371/journal.pone.0034723

Figure 5. Spatially separating Gag-Pol-Nef and gp120 alters Gag- and Pol-specific CD8+ T cell responses.

Figure 5

BALB/c mice (n = 6 per group) were i.m. inoculated (+) in both legs with an equimolar mixture of MGag and PN; gp120 alone, or a mixture of MGag, PN and gp120, where the amount of gp120 was titrated (mixture: 57, 30, 10 µg). Further groups received plasmid DNAs spatially separated as a mixture of MGag and PN in the right leg and titrated amounts (separate: 57, 30, 10 µg) of gp120 in the left leg. After 12 days, spleen cells were isolated and tested for specific cellular immune responses by measuring IFNγ production after stimulation with Gag (black), Pol (grey) or Env (hatched) specific peptides. IFNγ production was determined by FACS analysis after intracellular staining of IFNγ as described in Fig. 3. Cell culture medium served as a negative control. Data shown are representative of two experiments.