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. 2012 Apr 11;7(4):e34723. doi: 10.1371/journal.pone.0034723

Figure 6. Effect temporal separation of Gag-Pol-Nef and gp120 administration on Gag- and Pol-specific CD8+ T cell responses.

Figure 6

BALB/c mice (n = 6 per group) were i.m. inoculated at day 0 with a mixture of MGag and PN; MGag, PN, and gp120; or MGag and PN, followed by a booster immunization with gp120 two weeks later (d 14); or vice versa, a mixture of MGag and PN, or MGag, PN and gp120 at day 14; or primed at day 0 with gp120 followed by a booster immunization with MGag and PN at day 14. At day 29, spleen cells were isolated and tested for specific cellular immune responses by measuring IFNγ production after stimulation with Gag (black), Pol (grey) or Env (hatched) specific peptides. IFNγ production was determined by FACS analysis after intracellular staining of IFNγ. Cell culture medium served as a negative control. Data shown are representative of two experiments.