Table 1. Summary of Antibiotic Inactivation Studies for Gram-positive Isolates.
Antibiotic | Resistant Strains | Number of antibiotic Inactivation Strains | Mechanism of Inactivation |
Apramycin | 7 | 0 | ------- |
Gentamicin | 5 | 0 | ------- |
Neomycin | 8 | 0 | ------- |
Streptomycin | 8 | 0 | ------- |
Tetracycline | 4 | 0 | ------- |
Minocycline | 1 | 0 | ------- |
Clindamycin | 6 | 0 | ------- |
Chloramphenicol | 12 | 0 | ------- |
Synercid | 2 | 0 | ------- |
Erythromycin | 7 | 1 | Phosphorylation |
Telithromycin | 4 | 4 | Phosphorylation Glycosylation |
Linezolid | 2 | 0 | ------- |
Novobiocin | 5 | 0 | ------- |
Cephalexin | 17 | 5 | Hydrolysis |
Ampicillin | 8 | 5 | Hydrolysis |
Piperacillin | 9 | 2 | Hydrolysis |
Daptomycin | 24 | 7 | Hydrolysis |
Strains were grown in 50% TSB for 5 days in presence of 20 µg/ml antibiotic. Conditional media was used for setting up disk diffusion assays and LC-MS analyses. Inactivation was defined as the absence of a zone of clearance around the disk. Hydrolytic mechanism of ß-lactam resistance is inferred.