Table 4. Kinetic parameters for MPH (2′)-II from B. faecium DSM 4810.

Substrate	Lactone Ring	Km (µM)	kcat (s−1)	Ki (µM)	kcat/Km(s−1 M−1)
Erythromycin1	14	38.2±6.24	0.192	-	5.03×103
Clarithromycin	14	42.0±6.26	0.286	-	6.81×103
Telithromycin	14	23.9±4.22	0.168	-	7.05×103
Roxithromycin	14	19.3±2.60	0.262	-	1.36×104
Azithromycin	15	60.6±8.86	0.312	-	5.18×103
Spiramycin	16	17.3±3.18	0.157	-	9.10×103
Tylosin	16	14.5±4.00	0.088	-	6.07×102
GTP2	-	16.0±1.3	0.203	-	1.27×104

1- GTP held at 200 µM for antibiotic substrates, 2-erythomycin held at 400 µM.