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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Immunol. 2012 Mar 12;188(8):3648–3657. doi: 10.4049/jimmunol.1101278

Figure 2.

Figure 2

Ethanol induced Nox mRNA and protein expression levels in vivo and in vitro. A, mAMs were collected from control (Con) and ethanol-fed (EtOH) mice. mRNA levels of Nox1, Nox2, Nox4, p22phox, p47phox and p67phox in these mAMs were measured (n=5). B, Cultured MH-S cells were either untreated (None) or ethanol-treated (EtOH, 0.08%) for 3 days. mRNA levels of Nox1, Nox2, Nox4, p22phox, p47phox and p67phox in these MH-S cells were measured (n=3 independent experiments). All mRNA values were measured by qRT-PCR, normalized to 9s mRNA, and expressed as mean ± SEM, relative to no treatment or control. C, Protein expression of Nox1, Nox2 and Nox4 in cultured MH-S cells either untreated (None) or ethanol-treated (EtOH, 0.08%) for 3 days. Protein values in these MH-S cells were assessed by Western blotting and densitometric analysis, normalized to GAPDH protein levels (n=3 independent experiments), and expressed as mean ± SEM, relative to no treatment. *p<0.05, EtOH vs. Con or None.