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Total ion chromatograms (TIC) of semastatin modified by DEPC at a peptide:DEPC ratio of 1:4 with TCEP added after the reaction with DEPC (a) Products after allowing the peptide to remain in solution for 10 min after TCEP addition and (b) 2 h after adding TCEP. Chromatographic peaks notated with a residue(s) having an asterisk indicate that the corresponding chromatographic peak is the peptide labeled at only the indicated residue(s).
