Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Traffic. 2012 Feb 20;13(5):694–704. doi: 10.1111/j.1600-0854.2012.01335.x

Figure 2. Tyrosine 16 and glycine 17 are important for apicoplast targeting of APT1.

Figure 2

Amino acids 15–36 were changed to alanine either individually or in combination and the mutant proteins were expressed in T. gondii bearing either the apicoplast marker or the Golgi marker as indicated. IFA was conducted as in Figure 1. Labels above the grayscale panels indicate the color shown in the merged images. IFAs of APT1(G17A)-HA with GRASP55 and the triple mutant APT1(T18A,S20A,T21A)-HA were performed with stable transfectants; the remainder are from transient expression. Bar = 2 µM.