Table 2.
Altered expression of genes associated with cell lysis in CPV treated S. aureus COL cells
Locus ID | Genea | Gene/Protein Name | Sub-functional Category | Fold Change |
---|---|---|---|---|
Cell envelope | ||||
Downregulated Genes | ||||
SACOL0938 | dltD | DltD protein | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −10.41 |
SACOL0054 | NA | Mur ligase family protein, authentic frameshift | Biosynthesis and degradation of murein sacculus and peptidoglycan | −9.61 |
SACOL0937 | dltC | D-alanyl carrier protein | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −5.99 |
SACOL0936 | dltB | DltB protein | −5.41 | |
SACOL0247 | lrgA | holin-like protein LrgA | Biosynthesis and degradation of murein sacculus and peptidoglycan | −4.64 |
SACOL0052 | NA | glycosyl transferase, group 1 family protein | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −4.19 |
SACOL1195 | mraY | phospho-N-acetylmuramoyl-pentapeptide-transferase | Biosynthesis and degradation of murein sacculus and peptidoglycan | −3.71 |
SACOL0697 | tagX | teichoic acid biosynthesis protein X | −3.51 | |
SACOL0801 | murB | UDP-N-acetylenolpyruvoylglucosamine reductase | −2.97 | |
SACOL1023 | murE | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | −2.89 | |
SACOL1194 | pbp1 | penicillin-binding protein 1 | −2.5 | |
SACOL1196 | murD | UDP-N-acetylmuramoylalanine--D-glutamate ligase | −2.45 | |
SACOL2074 | NA | D-alanine--D-alanine ligase | −2.39 | |
SACOL1687 | NA | N-acetylmuramoyl-L-alanine amidase, family 3 | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −2.38 |
SACOL0543 | glmU | UDP-N-acetylglucosamine pyrophosphorylase | −2.21 | |
SACOL2103 | NA | UDP-N-acetylglucosamine 2-epimerase | −2.21 | |
SACOL0033 | mecA | penicillin-binding protein 2 | Biosynthesis and degradation of murein sacculus and peptidoglycan | −2.15 |
SACOL1329 | femC | glutamine synthetase FemC | −2.15 | |
SACOL0242 | NA | teichoic acid biosynthesis protein, putative | −2.12 | |
SACOL1134 | kdtB | lipopolysaccharide core biosynthesis protein KdtB | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −2.09 |
SACOL1609 | pbp3 | penicillin-binding protein 3 | Biosynthesis and degradation of murein sacculus and peptidoglycan | −2.01 |
SACOL1453 | murG | UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase | −1.98 | |
SACOL0263 | lytM | peptidoglycan hydrolase | −1.96 | |
SACOL0695 | NA | tagG protein, teichoic acid ABC transporter protein, putative | −1.87 | |
SACOL1066 | NA | fmt protein | −1.83 | |
SACOL1062 | atl | bifunctional autolysin | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | −1.78 |
SACOL1424 | NA | phosphate ABC transporter, phosphate-binding protein | Other | −1.74 |
SACOL1790 | murC | UDP-N-acetylmuramate--alanine ligase | Biosynthesis and degradation of murein sacculus and peptidoglycan | −1.58 |
SACOL1951 | NA | Mur ligase family protein | −1.57 | |
Upregulated Genes | ||||
SACOL2571 | cwrA | conserved hypothetical protein | Conserved | 24.05 |
SACOL1434 | NA | alanine racemase family protein | Biosynthesis and degradation of murein sacculus and peptidoglycan | 21.26 |
SACOL0147 | cap5L | capsular polysaccharide biosynthesis protein Cap5L | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | 4.22 |
SACOL0150 | cap5O | capsular polysaccharide biosynthesis protein Cap5O | 4.02 | |
SACOL0148 | cap5M | capsular polysaccharide biosynthesis galactosyltransferase Cap5M | 3.98 | |
SACOL0151 | cap5P | UDP-N-acetylglucosamine 2-epimerase Cap5P | 3.61 | |
SACOL0149 | cap5N | capsular polysaccharide biosynthesis protein Cap5N | 3.55 | |
SACOL1161 | murI | glutamate racemase | Biosynthesis and degradation of murein sacculus and peptidoglycan | 3.11 |
SACOL0146 | cap5K | capsular polysaccharide biosynthesis protein Cap5K | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | 2.95 |
SACOL0144 | cap5I | capsular polysaccharide biosynthesis protein Cap5I | 2.83 | |
SACOL0145 | cap5J | capsular polysaccharide biosynthesis protein Cap5J | 2.79 | |
SACOL0140 | cap5E | capsular polysaccharide biosynthesis protein Cap5E | 2.67 | |
SACOL0141 | cap5F | capsular polysaccharide biosynthesis protein Cap5F | 2.17 | |
SACOL2092 | murAA | UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 | Biosynthesis and degradation of murein sacculus and peptidoglycan | 2.1 |
SACOL0143 | cap5H | capsular polysaccharide biosynthesis protein Cap5H | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | 2.04 |
SACOL0699 | pbp4 | penicillin-binding protein 4 | Biosynthesis and degradation of murein sacculus and peptidoglycan | 2.04 |
SACOL0142 | cap5G | UDP-N-acetylglucosamine 2-epimerase Cap5G | Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides | 1.99 |
SACOL0136 | cap5A | capsular polysaccharide biosynthesis protein Cap5A | 1.68 | |
SACOL0137 | cap5B | capsular polysaccharide biosynthesis protein Cap5B | 1.67 | |
SACOL2116 | murAB | UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 | Biosynthesis and degradation of murein sacculus and peptidoglycan | 1.67 |
Cellular processes | ||||
Downregulated Genes | ||||
SACOL0935 | dltA | D-alanine-activating enzyme/D-alanine-D-alanyl carrier protein ligase | Toxin production and resistance | −11.93 |
SACOL2295 | NA | staphyloxanthin biosynthesis protein, putative | Pathogenesis | −8.55 |
SACOL1535 | srrA | DNA-binding response regulator SrrA | −8 | |
SACOL0244 | scdA | ScdA protein | Cell division | −6.24 |
SACOL0270 | NA | staphyloxanthin biosynthesis protein, putative | Pathogenesis | −5.55 |
SACOL0095 | spa | immunoglobulin G binding protein A precursor | −5.54 | |
SACOL1437 | NA | cold shock protein, CSD family | Adaptations to atypical conditions | −4.87 |
SACOL2126 | luxS | autoinducer-2 production protein LuxS | Other | −4.15 |
SACOL0956 | kapB | kinase-associated protein B | Adaptations to atypical conditions | −3.8 |
SACOL1193 | NA | cell division protein FtsL | Cell division | −3.6 |
SACOL0608 | sdrC | sdrC protein | Cell adhesion | −3.53 |
SACOL1328 | glnR | glutamine synthetase repressor | Toxin production and resistance | −3.35 |
SACOL2291 | NA | staphyloxanthin biosynthesis protein | Pathogenesis | −3.33 |
SACOL0552 | NA | general stress protein 13 | Adaptations to atypical conditions | −3.32 |
SACOL1534 | srrB | sensor histidine kinase SrrB | Pathogenesis | −3.27 |
SACOL0743 | bacA | bacitracin resistance protein | Toxin production and resistance | −3.23 |
SACOL1396 | fmtC | fmtC protein | −3.18 | |
SACOL2075 | ftsW | cell division protein, FtsW/RodA/SpoVE family | Cell division | −3.12 |
SACOL1197 | divIB | cell division protein | −2.91 | |
SACOL0766 | saeR | DNA-binding response regulator SaeR | Pathogenesis | −2.87 |
SACOL1205 | NA | cell-division initiation protein, putative | Cell division | −2.86 |
SACOL0452 | ahpC | alkyl hydroperoxide reductase, C subunit | Detoxification | −2.8 |
SACOL1537 | scpB | segregation and condensation protein B | Cell division | −2.72 |
SACOL1624 | era | GTP-binding protein Era | −2.46 | |
SACOL1538 | scpA | segregation and condensation protein A | −2.31 | |
SACOL1184 | NA | exfoliative toxin, putative | Toxin production and resistance | −2.14 |
SACOL0746 | norR | transcriptional regulator, MarR family | −2.06 | |
SACOL2731 | NA | cold shock protein, CSD family | Adaptations to atypical conditions | −2.06 |
SACOL1383 | mscL | large conductance mechanosensitive channel protein | −1.96 | |
SACOL2024 | agrD | accessory gene regulator protein D | Pathogenesis | −1.91 |
SACOL0610 | sdrE | sdrE protein | Cell adhesion | −1.89 |
SACOL1410 | femA | femA protein | Toxin production and resistance | −1.76 |
SACOL0765 | saeS | sensor histidine kinase SaeS | Pathogenesis | −1.69 |
SACOL0245 | lytS | sensor histidine kinase LytS | −1.66 | |
SACOL1198 | ftsA | cell division protein FtsA | Cell division | −1.53 |
Upregulated Genes | ||||
SACOL1943 | vraS | sensor histidine kinase VraS | Toxin production and resistance | 5.22 |
SACOL1450 | arlS | sensor histidine kinase ArlS | Pathogenesis | 4.74 |
SACOL2353 | tcaR | transcriptional regulator TcaR | Toxin production and resistance | 4.18 |
SACOL0672 | sarA | staphylococcal accessory regulator A | 4.11 | |
SACOL2157 | NA | drug resistance transporter, EmrB/QacA subfamily | 3.75 | |
SACOL1451 | arlR | DNA-binding response regulator ArlR | Pathogenesis | 3.61 |
SACOL2289 | sarY | staphylococcal accessory regulator Y | Toxin production and resistance | 3.21 |
SACOL2258 | sarV | staphylococcal accessory regulator V | 2.87 | |
SACOL1942 | vraR | DNA-binding response regulator VraR | 2.61 | |
SACOL1003 | NA | negative regulator of competence MecA, putative | DNA transformation | 2.01 |
SACOL0608 | sdrC | sdrC protein | Cell adhesion | 1.82 |
Protein fate | ||||
Downregulated Genes | ||||
SACOL1801 | NA | peptidase, M20/M25/M40 family | Degradation proteins, peptides, and glycopeptides | −3.45 |
SACOL0806 | pepT | peptidase T | −2.89 | |
SACOL2385 | NA | heat shock protein, Hsp20 family | Protein folding and stabilization | −2.77 |
SACOL1946 | NA | methionine aminopeptidase, type I | Protein modification and repair | −2.62 |
SACOL1777 | NA | serine protease HtrA, putative | Degradation proteins, peptides, and glycopeptides | −2.42 |
SACOL0581 | secE | preprotein translocase, SecE subunit | Protein and peptide secretion and trafficking | −2.13 |
SACOL1588 | NA | proline dipeptidase | Degradation proteins, peptides, and glycopeptides | −2.12 |
SACOL2038 | NA | metalloendopeptidase, putative, glycoprotease family | −2.06 | |
Upregulated Genes | ||||
SACOL0979 | clpB | ATP-dependent Clp protease, ATP-binding subunit ClpB | Degradation proteins, peptides, and glycopeptides | 9.42 |
SACOL1433 | NA | peptidase, M20/M25/M40 family | 8.7 | |
SACOL2125 | NA | peptidase, M20/M25/M40 family | 7.13 | |
SACOL0570 | clpC | ATP-dependent Clp protease, ATP-binding subunit ClpC, authentic frameshift | 5.92 | |
SACOL1636 | dnaJ | dnaJ protein | Protein folding and stabilization | 4.94 |
SACOL0833 | clpP | ATP-dependent Clp protease, proteolytic subunit ClpP | Degradation proteins, peptides, and glycopeptides | 3.72 |
SACOL1638 | grpE | heat shock protein GrpE | Protein folding and stabilization | 3.5 |
SACOL0968 | spsA | signal peptidase IA, inactive | Protein and peptide secretion and trafficking | 2.98 |
SACOL1637 | dnaK | dnaK protein | Protein folding and stabilization | 2.67 |
SACOL2438 | NA | endopeptidase, putative | Degradation proteins, peptides, and glycopeptides | 2.46 |
SACOL1459 | NA | peptide methionine sulfoxide reductase, degenerate | Protein modification and repair | 2.38 |
SACOL2016 | groEL | chaperonin, 60 kDa | Protein folding and stabilization | 2.36 |
SACOL0556 | NA | chaperonin, 33 kDa | 2.17 | |
SACOL0844 | secG | preprotein translocase, SecG subunit | Protein and peptide secretion and trafficking | 2.13 |
SACOL0969 | spsB | signal peptidase IB | 2.02 | |
SACOL2017 | groES | chaperonin, 10 kDa | Protein folding and stabilization | 1.81 |
NA – Gene symbol not available