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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Traffic. 2012 Mar 14;13(5):727–744. doi: 10.1111/j.1600-0854.2012.01343.x

Fig. 3. Golgi stacks in Rab6-depleted cells retain a polarized distribution of resident Golgi proteins along the cis-trans axis.

Fig. 3

Wild-type HeLa cells incubated with either scrambled (siControl, A) or Rab6 siRNA (siRab6, B) for 96 h were fixed for indirect immunofluorescence localization using antibodies specific for p115 and β-galactosyltransferase (GalT). Images shown are maximum intensity projections of confocal image stacks. (C) HeLa cell cultures were processed by Western blot analysis to confirm the efficiency of Rab6 protein knockdown. Bar, 10 μm (A and B).