Figure 1. Ski is expressed in cartilage.

Chick tissues including lung, brain, heart, liver and sternal cartilage were harvested and proteins were extracted. Fifty μg quantities of solubilized protein were loaded and run on polyacrylamide gels, transferred to nylon membranes and Western analyses were performed to detect Ski, with detection of β-actin confirming equal loading of the gel. The analysis was performed 3 times, with the representative blot shown.