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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Biochem. 2012 Jun;113(6):2156–2166. doi: 10.1002/jcb.24089

Figure 5. Ski stimulates Runx2 expression and reverses the effect of TGF-β on cyclin D1 and Runx2 expression.

Figure 5

Chick USCs were isolated and cultured in medium containing 50 μg/mL ascorbic acid for 24 hours. Following this, cells were transfected with cmv-Ski (Ski) or an empty vector (Control). Twenty four hours post-transfection, cultures were treated with or without 5 ng/mL TGF-β for 24 additional hours. mRNA and total cellular protein were harvested and qPCR and Western blotting was performed to quantify cyclin D1 mRNA levels (A) and both cyclin D1 (Lamin B load control) and Runx2 (β-actin load control) protein levels (B). In panel (A), statistically significant differences were identified via ANOVA (N=3, p<0.05) with asterisks (*) denoting significance from the control value and double asterisks (**) denoting significance from the TGF-β-treated group. Blots shown in panel (B) are representative of experiments that were repeated 3 times.