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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Cell Biochem. 2012 Jun;113(6):2156–2166. doi: 10.1002/jcb.24089

Figure 8. Induction of colX and osteocalcin expression by Ski is blocked by HDAC inhibition.

Figure 8

Chick USCs were isolated and cultured in medium containing 50 μg/mL ascorbic acid. Twenty four hours after plating, cells were pre-treated with the HDAC inhibitor suberoylanilidehydroxamic acid (SAHA, 5μM). Following this, cells were transfected with cmv-Ski (Ski) or empty vector (Control) and maintained in culture for an additional 24 hours in the presence of SAHA. Messenger RNA was then harvested and qPCR was performed to quantify colX (A) and osteocalcin (B) expression levels. For both panels, statistically significant differences were identified via ANOVA (N=3, p<0.05) with asterisks (*) denoting significance from the control value for each gene assessed and double asterisks (**) denoting significance from the Ski-transfected group.