A, The given cell lines were treated with 5μM API-1 for 24 h. B, H157 cells were treated with different concentrations of API-1 as indicated for 12 h. C, H157 cells were treated with 5μM API-1 for the indicated times. After the aforementioned treatments, the cells were subjected to preparation of whole-cell protein lysates and subsequent Western blot analysis for the given proteins. LE, longer exposure.