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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Immunol. 2012 Mar 16;188(8):3869–3875. doi: 10.4049/jimmunol.1103627

Figure 2.

Figure 2

TIGIT cell-intrinsic signaling inhibits T cell proliferation. (A) Proliferation of human CD4+ T cells labeled with CFSE and activated with anti-CD3 and anti-CD28 with plate-bound agonistic anti-TIGIT or isotype-matched control antibody. On day 5, CFSE dilution was analyzed by flow cytometry after gating on viable cells. (B) Percentages of dividing cells after activation represent the CFSElow cells (n=8). Mean values are shown ± SD. (C) Viability was assessed in all the experiments. (D) No significant differences were observed in the frequency of viable cells. (E) Flow cytometry of the surface expression of CD25 and CD69 as markers of activation of human CD4+ T cells activated in presence of agonistic anti-TIGIT or isotype control, assessed on day 5 after activation. (F) Sorting strategy for memory T cells (CD4+ CD62L+ CD25low CD45RO+) purity. (G) CFSE proliferation assay of memory T cells in the presence of agonistic anti-TIGIT. Proliferation was assessed on day 5 after gating on viable cells. Percentages of proliferating cells are depicted above the histograms. (H) Quantitative real-time RT-PCR analysis of relative IL-2 gene expression in FACS-sorted memory T cells. Data shown are representative of four different donors.