Dampening of observed F
v/F
m with changing emission band position and width. The plots show the average of F
v/F
m(λex,λem) measured in all a algal cultures, with λex = 470 nm, and b cyanobacterial cultures, with λex = 590 nm. Before averaging, F
v/F
m(λex,λem) emission spectra were normalized to their peak (found in the 680–690 nm emission region). Dashed lines indicate the standard deviation of the normalized F
v/F
m(λex,λem) emission spectra. All lines were smoothed over 5 nm. The sharply peaked F
v/F
m feature observed in all cyanobacteria cultures imposes strict limitations on the configuration of the emission slit