Figure 2. Chronic rapamycin treatment decreases insulin signaling in skeletal muscle and liver.
Mice were treated with vehicle or 2.5 mg/kg rapamycin for 14 days, fasted for 12h and then injected with vehicle or 0.6 U/kg insulin 10 min before sacrifice. Insulin signaling in (A) skeletal muscle and (B) liver. (C) Gene expression in the soleus from fed mice. All values are presented as mean ± SD. n=6–10, *P<0.05 and **P<0.01.