FIG 7 .

FdeC_B prevents the spread of UPEC infection into the kidney. Mice were immunized intranasally with a 10:1 ratio of FdeC_B to CT containing 100 µg of FdeC_B or with 10 µg CT alone (mock immunized). After two boosts of 25 µg antigen (10:1 ratio of antigen to CT) or CT alone given on days 7 and 14, the individual mice were transurethrally challenged with 10⁸ CFU of UPEC strain 536 (A) and CFT073 (B). After 48 h, bladder and kidneys were harvested and homogenized. Bacteria in urine and in the tissue homogenates were enumerated by plating serial dilutions. Symbols represent CFU/g tissue or CFU/ml urine of individual mice, and bars indicate median values. P values were determined using the nonparametric Mann-Whitney significance test.