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. 2001 May 1;98(10):5538–5543. doi: 10.1073/pnas.091603098

Figure 1.

Figure 1

The purified Xrcc3⋅Rad51C and Rad51C proteins. (A) SDS/12% PAGE of Ni column fractions of Xrcc3⋅Rad51C. Fractions (3 μl) containing Xrcc3⋅Rad51C were analyzed by SDS/12% PAGE. Fraction numbers are indicated at the top of the gel. (B) SDS/12% PAGE of the purified Xrcc3⋅Rad51C and Rad51C proteins. Lane 2 is Xrcc3⋅Rad51C (0.5 μg) eluted from an Affi-Gel-heparin column, and lane 3 is Rad51C (0.5 μg) eluted from Ni nitrilotriacetate agarose. Lane 1 is molecular mass markers. The proteins were visualized by Coomassie brilliant blue staining. (C) Elution profiles of Xrcc3⋅Rad51C and HsRad51 in Superdex 200 h chromatography. Xrcc3⋅Rad51C (20 μg) and HsRad51 (20 μg) were analyzed by Superdex 200 h column chromatography. (Upper) Elution profile of HsRad51. (Lower) Elution profile of Xrcc3⋅Rad51C.