Figure 2.

DNA-binding activity of Xrcc3⋅Rad51C. (A) The ssDNA binding of Xrcc3⋅Rad51C. A ³²P-labeled single-stranded 50-mer oligonucleotide (100 nM), which does not contain intramolecular base pairing, was incubated with Xrcc3⋅Rad51C at 37°C for 10 min, and the reactions were analyzed by nondenaturing 8% PAGE in TBE buffer. Concentrations of Xrcc3⋅Rad51C were 55 nM (lane 2), 110 nM (lane 3), and 220 nM (lane 4) and were calculated as heterodimers. (B) The dsDNA binding of Xrcc3⋅Rad51C. A ³²P-labeled double-stranded 50-mer oligonucleotide (200 nM) was used as the substrate. Concentrations of Xrcc3⋅Rad51C were 55 nM (lane 2), 110 nM (lane 3), and 220 nM (lane 4) and were calculated as heterodimers.