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. 2001 May 1;98(10):5538–5543. doi: 10.1073/pnas.091603098

Figure 6.

Figure 6

Homologous-pairing activity of Rad51C. A 32P-labeled single-stranded 50-mer oligonucleotide (1 μM) was incubated with Xrcc3⋅Rad51C in the standard reaction mixture at 37°C for 5 min, and the reactions were initiated by the addition of 12 mM MgCl2 and pGsat4 form I DNA (13 μM). The reactions were continued for 20 min. Lane 1 is the negative control without protein. Lanes 2–4 are the experiments with Xrcc3⋅Rad51C, and lanes 5–7 are the experiments with Rad51C alone. The concentrations of Xrcc3⋅Rad51C and Rad51C used in the DNA-binding experiments were 0.2 μM (lanes 2 and 5) and 0.6 μM (lanes 3, 4, 6, and 7). Lanes 4 and 7 are the negative controls with heterologous ssDNA and dsDNA. The concentration of Xrcc3⋅Rad51C was calculated as heterodimers.

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