FIG. 13.
Rescue of oxidative stress induced DNA damage in fibroblast–MCF7 cell cocultures with NAC and L-NAME. Treatment with NAC and L-NAME abolishes DNA double-strand breaks in coculture. Day-2 cocultures of hTERT fibroblasts and MCF7 cells were treated with 10 mM NAC or with 20 mM L-NAME or with vehicle alone (H2O) for 72 h. Then, cells were immunostained with anti-gamma-H2AX (red) antibodies. DAPI was used to stain nuclei (blue). Upper panels show only the red channel to appreciate gamma-H2AX staining, while the lower panels show nuclei staining. High levels of DNA double-strand breaks are detected in cocultured cells. Note that treatment with NAC and L-NAME abolishes DNA double-strand breaks in cocultured cells. Importantly, images were acquired using identical exposure settings. Original magnification, 40×. Modified and reproduced with permission from Martinez-Outschoorn et al. (66). (To see this illustration in color, the reader is referred to the Web version of this article at www.liebertonline.com/ars).