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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: J Immunol Methods. 2012 Jan 16;377(1-2):15–22. doi: 10.1016/j.jim.2011.12.009

Figure 1. Reactivity of HD mAbs to human and mouse DEC205 on transfectant cells.

Figure 1

(A) Two of the 10 chosen HD mAbs, HD30 and HD83, were compared with anti-hDEC205 MG38.2 and anti-mDEC205 NLDC145 mAbs for their reactivities to human and mouse DEC205. CHO cells stably expressing hDEC205 or mDEC205 were stained with HD30, HD83, cloned MG38.2 (MG38.2 with the constant regions of mouse IgG1 heavy chains), or cloned NLDC145 (NLDC145 with the constant regions of mouse IgG1 heavy chains). All antibodies were used at 1 µg/ml followed by detection with PE-conjugated anti-mIgG1. Mouse IgG1 antibody was used as a negative isotype control.

(B) Reactivities of the 10 chosen HD mAbs to human and mouse DEC205 were compared by FACS analysis with CHO/hDEC205 and CHO/mDEC205 cells as in (A) but binding of each mAb to the cells was detected with PE-conjugated anti-mIgG.