Skip to main content
. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: J Immunol Methods. 2012 Jan 16;377(1-2):15–22. doi: 10.1016/j.jim.2011.12.009

Figure 2. Western blot analysis of anti-DEC205 mAbs.

Figure 2

(A) Control CHO, CHO/mDEC205, and CHO/hDEC205 cells were lysed and each lysate equivalent to 5 × 105 cells was subjected to the western blot analyses by HD30, HD83, NLDC145, and anti-actin antibodies.

(B) A series of deletion constructs in the extracellular domain of hDEC205 were generated in fusion with hIgG1 Fc domain, and each deletion construct was expressed in 293T cells. Lysates of cells expressing different subdomains of hDEC205 were subjected to western blot analyses with HD30, HD83, or anti-hIgG.