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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: J Immunol Methods. 2012 Jan 16;377(1-2):15–22. doi: 10.1016/j.jim.2011.12.009

Figure 3. Immunohistochemical stains of HD mAbs on the splenic tissues from human and monkey.

Figure 3

(A) Cells expressing DEC205 at high levels were detected in the white pulp of the human spleen by HD30. Double staining of human spleen sections was carried out with anti-DEC205 mAb HD30 (in green) and anti-CD11c antibody (DCs in red).

(B) HD83 stained DEC205+ cells in the T-cell areas of the white pulps in monkey spleen. Double staining of monkey spleen sections was carried out with anti-DEC205 mAb HD83 (in red) and antibodies to CD206 (red pulp macrophages in green; upper panel) or CD8 (T cells in green; lower panel).