Table 1. Summary of mouse Ig isotype, species reactivity, and epitope mapping for the 10 chosen HD mAbs.

Reactivity of anti-hDEC205 and anti-mDEC205 was determined on the basis of relative intensity of FACS staining on CHO/hDEC205 and CHO/mDEC205 cells (−, not detectable; +, weaker than anti-hDEC205 MG38.2 or anti-mDEC205 NLDC145; ++, similar to or stronger than MG38.2 or NLDC145). Reactivity of anti-monkey DEC205 was noted as positive (+) or negative (−) based on the result of immunohistochemical staining of monkey splenic tissue sections.

mAb	Isotype	Anti-human DEC205	Anti-mouse DEC205	Anti-monkey DEC205	Epitope-mapped Subdomain
HD20	IgG1, kappa	++	++	−	CR-FN
HD24	IgG1, kappa	++	++	−	CR-FN
HD30	IgG1, kappa	++	−	+	CRD1–2
HD51	IgG1, kappa	++	−	+	CRD1–2
HD71	IgG2b, kappa	++	++	+	CRD3–4
HD73	IgG1, kappa	++	+	+	CR-FN
HD77	IgG1, kappa	++	+	+	CRD1–2
HD83	IgG1, kappa	++	++	+	CRD1–2
HD106	IgG1, kappa	++	−	+	CRD1–2
HD109	IgG1, kappa	++	++	+	CRD3–4