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. 2012 Apr 12;2:362. doi: 10.1038/srep00362

Figure 1. Clonogenic death of FSaII and MCF-7 cells treated with metformin alone or with radiation.

Figure 1

(A, B) Cells were incubated for 1–48 h with 0.5–10 mM metformin and the clonogenic survival was determined. (C) Cells were cultured in media containing 5–100 µM of metformin for 10 days for FSaII cells and 20 days for MCF-7 days. (D, E) Cells were incubated with 1 or 5 mM metformin for 24 h, irradiated with various doses of X-rays, further incubated with metformin for 24 h and then the clonogenic survival was determined. The radiation survival curves were normalized for the cell death caused by metformin alone. All data points are means of 5–7 independent experiments ± 1 S.E.