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Downstream DNA is dispensable for promoter escape by RNA polymerase II under conditions that bypass the requirement for TFIIH in promoter escape. (A) Schematic diagram of reaction protocol. (B) AdML (lanes 1–3) or Ad(−9/+9) (lanes 4–7) DNA templates were incubated for 20 min at 30°C with or without 5 units of HindIII before the assembly of preinitiation complexes. Promoter escape assays were performed, with or without TFIIH, as described in the legend to Fig. 2.
