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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: J Proteome Res. 2012 Feb 21;11(3):1633–1643. doi: 10.1021/pr2008384

Figure 4.

Figure 4

Ethanol consumption and/or SIRT3 KO lead to a significant increase in the acetylation of IDH2, SOD2 and ALDH2. Standard immunoblotting reveals a significant increase in IDH2 expression in ethanol-fed groups. SOD2 and ALDH2 expression was only significantly increased in the SIRT3KO, ethanol-consuming mice. Bands were normalized to VDAC and are expressed as a % of WT control. Immunoprecipitation with anti-acetyl-lys antibodies reveals varying altered acetylation patterns of IDH2, SOD2 and ALDH2. Bands were quantified, adjusted for protein expression and are presented as a % of WT control. * P < 0.01 from WT control; $ P < 0.01 from KO control; # P < 0.01 from WT ethanol.