Table 1.
Ethanol consumption and SIRT3 ablation reveals numerous targets of mitochondrial protein acetylation.
| Protein ID | Accession # |
WT Cont |
WT EtOH |
KO Cont |
KO EtOH |
Biological Process |
|---|---|---|---|---|---|---|
| Ornithine aminotransferase | P29758 | 0 | 1 | 1 | 2 | Amino Acid Biosynthesis |
| ATP synthase subunit alpha | Q03265 | 0 | 1 | 0 | 5 | ATP Biosynthesis |
| NADH dehydrogenase (ubiquinone) 1 alpha | Q99LC3 | 0 | 1 | 1 | 1 | Electron Transport Chain |
| Calreticulin | P14211 | 0 | 0 | 0 | 1 | Protein Folding |
| Stress-70 protein | P38647 | 1 | 1 | 1 | 1 | Protein Folding |
| Heat shock cognate 71 kDa protein | P63017 | 1 | 1 | 1 | 1 | Stress Response/Protein Folding |
| Hypoxia up-regulated protein 1 | Q9JKR6 | 0 | 1 | 1 | 1 | Stress Response/Protein Folding |
| Electron transfer flavoprotein subunit alpha | Q99LC5 | 0 | 2 | 1 | 4 | Electron Transport Chain |
| Electron transfer flavoprotein subunit beta | Q9DCW4 | 0 | 3 | 2 | 3 | Electron Transport Chain |
| Beta-lactamase-like protein 2 | Q99KR3 | 0 | 1 | 0 | 1 | Not reported |
| Valacyclovir hydrolase | Q8R164 | 0 | 0 | 0 | 1 | Not reported |
| Peroxisomal coenzyme A diphosphatase | Q99P30 | 0 | 3 | 0 | 3 | Acetyl-CoA Catabolic Process |
| 3-hydroxyisobutyryl-CoA hydrolase | Q8QZS1 | 1 | 1 | 1 | 1 | Amino Acid Catabolism |
| Fumarylacetoacetase | P35505 | 0 | 1 | 1 | 1 | Amino Acid Catabolism |
| Epoxide Hydrolase 2 | P34914 | 1 | 1 | 1 | 1 | Aromatic Hydrocarbon Catabolism |
| ATP synthase subunit beta | P56480 | 1 | 1 | 1 | 3 | ATP Biosynthesis |
| Acyl coenzyme A thioesterase 13 | Q9CQR4 | 0 | 1 | 1 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Bile acid-CoA:amino acid N-acyltransferase | Q91X34 | 0 | 1 | 1 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Fructose-1,6-bisphosphatase 1 | Q9QXD6 | 0 | 1 | 1 | 1 | Gluconeogenesis |
| Cathepsin B | P10605 | 0 | 0 | 0 | 1 | Regulation of Catalytic Activity |
| ATP synthase subunit d | Q9DCX2 | 2 | 2 | 2 | 2 | ATP Biosynthesis |
| Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase | O35459 | 1 | 1 | 1 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Alpha-methylacyl-CoA racemase | O09174 | 1 | 1 | 1 | 1 | Isoprenoid Catabolic Process |
| Adenylate kinase 2 | Q9WTP6 | 0 | 1 | 1 | 2 | ATP Biosynthesis |
| Acyl-CoA synthetase family member 2 | Q8VCW8 | 0 | 0 | 0 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Propionyl-CoA carboxylase alpha chain | Q91ZA3 | 0 | 0 | 0 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Pyruvate carboxylase | Q05920 | 1 | 2 | 1 | 3 | Gluconeogenesis/Lipid Metabolism |
| Succinyl-CoA ligase [ADP-forming] subunit beta | Q9Z219 | 0 | 1 | 1 | 1 | Proteolysis |
| Succinyl-CoA ligase [GDP-forming] subunit beta | Q9Z218 | 0 | 1 | 1 | 1 | Proteolysis |
| Carbamoyl phosphate synthase | Q8C196 | 1 | 2 | 1 | 3 | Urea Cycle |
| Arginosuccinate sythase | P16460 | 0 | 1 | 1 | 1 | Urea Cycle/Amino Acid Biosynthesis |
| Citrate Lyase subunit beta-like protein | Q8R4N0 | 1 | 1 | 1 | 1 | Cellular Aromatic Compound Metabolic Process |
| 2-hydroxyacyl-CoA lyase 1 | Q9QXE0 | 1 | 1 | 1 | 2 | Fatty Acid Metabolism |
| Enoyl-CoA hydratase | Q8BH95 | 0 | 1 | 0 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Enoyl-CoA hydratase domain-containing protein 2 | Q3TLP5 | 0 | 1 | 1 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Glutamine synthetase | P15105 | 0 | 1 | 1 | 1 | Glutamine Biosynthetic Process |
| Carbonic anhydrase 3 | P16015 | 0 | 2 | 2 | 2 | One-Carbon Metabolic Process |
| Aconitase hydratase | Q99KI0 | 0 | 0 | 0 | 2 | TCA Cycle |
| Cytochrome b-c1 complex subunit 1 | Q9CZ13 | 1 | 2 | 2 | 3 | Electron Transport/Respiratory Chain |
| Myelin basic protein | P04370 | 1 | 2 | 1 | 2 | Myelination |
| 2-oxoisovalerate dehydrogenase subunit beta | Q6P3A8 | 0 | 1 | 0 | 1 | Amino Acid Catabolism |
| 4-hydroxyphenylpyruvate dioxygenase | P49429 | 1 | 1 | 1 | 1 | Amino Acid Catabolism |
| Dimethylglycine dehydrogenase | Q9DBT9 | 2 | 2 | 2 | 2 | Amino Acid Catabolism/Oxidation-Reduction |
| Sarcosine dehydrogenase | Q99LB7 | 1 | 1 | 1 | 2 | Amino Acid Catabolism/Oxidation-Reduction |
| Superoxide dismutase [Mn] | P09671 | 1 | 1 | 1 | 1 | Antioxidant |
| Glutamate dehydrogenase 1 | P26443 | 3 | 3 | 3 | 4 | Cellular Amino Acid Metabolism |
| NADH-cytochrome b5 reductase 3 | Q9DCN2 | 0 | 1 | 1 | 1 | Cholesterol Biosynthesis/Lipid Metabolism |
| Dihydrolipoyl dehydrogenase | O08749 | 1 | 1 | 1 | 1 | Electron Transport |
| Cytochrome b-c1 complex subunit Rieske | Q9CR68 | 0 | 0 | 0 | 1 | Electron Transport Chain |
| Electron transfer flavoprotein-ubiquinone oxidoreductase | Q921G7 | 0 | 0 | 0 | 1 | Electron Transport Chain |
| Succinate dehydrogenase [ubiquinone] flavoprotein | Q8K2B3 | 0 | 1 | 0 | 1 | Electron Transport Chain/TCA Cycle |
| NADH dehydrogenase [ubiquinone] flavoprotein 2 | Q9D6J6 | 0 | 1 | 0 | 1 | Electron Transport/Respiratory Chain |
| Hydroxyacyl-coenzyme A dehydrogenase | Q61425 | 1 | 2 | 1 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Long-chain specific acyl-CoA dehydrogenase | P51174 | 1 | 2 | 2 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Medium-chain specific acyl-CoA dehydrogenase | P45952 | 4 | 4 | 4 | 4 | Fatty Acid Metabolism/Lipid Metabolism |
| Peroxisomal acyl-coenzyme A oxidase | Q9R0H0 | 1 | 3 | 2 | 5 | Fatty Acid Metabolism/Lipid Metabolism |
| Short-chain specific acyl-CoA dehydrogenase | Q07417 | 2 | 2 | 2 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Glycerol-2-phosphate dehydrogenase | P13707 | 0 | 1 | 1 | 1 | Gluconeogenesis |
| Pyruvate dehydrogenase E1 component subunit beta | Q9D051 | 1 | 1 | 1 | 1 | Glycolysis |
| Coproporphyrinogen-III oxidase | P36552 | 1 | 1 | 1 | 1 | Heme Biosynthesis/Porphyrin Biosynthesis |
| Aldehyde dehydrogenase | P47738 | 4 | 5 | 5 | 6 | Oxidation-Reduction Process |
| Alpha-aminoadipic semialdehyde dehydrogenase | Q9DBF1 | 1 | 1 | 1 | 2 | Oxidation-Reduction Process |
| Delta-1-pyrroline-5-carboxylate dehydrogenase | Q8CHT0 | 0 | 1 | 1 | 2 | Oxidation-Reduction Process |
| Hydroxyacid-oxoacid transhydrogenase | Q8R0N6 | 1 | 1 | 1 | 1 | Oxidation-Reduction Process |
| Isovaleryl-CoA dehydrogenase | Q9JHI5 | 1 | 1 | 1 | 1 | Oxidation-Reduction Process |
| Methylmalonate-semialdehyde dehydrogenase | Q9EQ20 | 1 | 1 | 1 | 1 | Oxidation-Reduction Process |
| Uricase | P25688 | 3 | 4 | 4 | 6 | Purine Metabolism |
| Isocitrate dehydrogenase [NAD] subunit apha | Q9D6R2 | 1 | 1 | 1 | 1 | TCA Cycle |
| Malate dehydrogenase | P14152 | 1 | 1 | 1 | 1 | TCA Cycle |
| Isocitrate dehydrogenase [NADP] | P54071 | 2 | 2 | 2 | 2 | TCA Cycle/Stress Response |
| Dihydropteridine reductase | Q8BVI4 | 0 | 1 | 1 | 1 | Tetrahydrobiopterin Biosynthesis |
| Peroxisomal bifunctional enzyme | Q9DBM2 | 0 | 1 | 1 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Peroxisomal multifunctional enzyme type 2 | P51660 | 1 | 1 | 2 | 2 | Fatty Acid Metabolism/Lipid Metabolism |
| Trifunctional enzyme subunit alpha | Q8BMS1 | 0 | 1 | 1 | 1 | Fatty Acid Metabolism/Lipid Metabolism |
| Catalase | P24270 | 0 | 1 | 0 | 5 | Antioxidant |
| Glutathione peroxidase 1 | P11352 | 0 | 1 | 1 | 1 | Antioxidant |
| Peroxiredoxin-1 | P35700 | 0 | 0 | 0 | 2 | Antioxidant |
| Voltage-dependent anion-selective channel protein 1 | Q60932 | 1 | 2 | 2 | 2 | Ion Transport |
| Betaine homocysteine S-methyltransferase | O35490 | 2 | 5 | 2 | 5 | Amino Acid Biosynthesis |
| Ornithine carbamoyltransferase | P11725 | 2 | 2 | 2 | 2 | Amino Acid Biosynthesis |
| Glutathione S-transferase Mu 1 | P10649 | 0 | 1 | 0 | 2 | Antioxidant |
| Hydroxymethylglutaryl-CoA synthase | P54869 | 1 | 3 | 2 | 6 | Cholesterol Biosynthesis/Lipid Metabolism |
| 3-ketoacyl-CoA thiolase | Q8BWT1 | 6 | 6 | 6 | 6 | Fatty Acid Metabolism/Lipid Metabolism |
| Acetyl-CoA acetyltransferase | Q8QZT1 | 6 | 6 | 6 | 6 | Ketone Body Metabolism |
| Aspartate aminotransferase | P05202 | 1 | 1 | 1 | 1 | Lipid Transport |
| non-specific lipid-transfer protein | P32020 | 0 | 2 | 2 | 4 | Lipid Transport |
| 4-aminobutyrate aminotransferase | P61922 | 0 | 0 | 0 | 1 | Neurotransmitter Catabolic Process |
| 3-Mercaptopyruvate sulfurtransferase | Q99J99 | 1 | 1 | 1 | 1 | Sulfate Transport |
| Thiosulfate sulfurtransferase | P52196 | 0 | 0 | 0 | 1 | Sulfate Transport |
| Citrate synthase | Q9CZU6 | 1 | 1 | 1 | 1 | TCA Cycle |
| 72 | 128 | 107 | 177 | |||