Table 2.
Overview of hepatic histological changes (extent of hepatic damage determined by serum ALT activity) induced by acetaminophen over time in mice and corresponding serum HMGB1 redox status.
| Time post-APAP treatmenta | 0 h | 5 h | 15 h | 24 h |
|---|---|---|---|---|
| Histological findings | No abnormalities detected necrosis with mild inflammatory cell infiltration | Centrilobular hepatocyte necrosis with moderate inflammatory cell infiltration (including neutrophil rolling) | Centrilobular hepatocyte detected | No abnormalities |
| Serum ALT (U/L)b | 31 ± 10 | 2641 ± 1,024c | 3,015 ± 801c | 3,811 ± 1,514c |
| Serum HMGB1 cysteine redox status identified | Molecule A | Molecule A + D | Molecule A + D | Molecule D |
a
The corresponding LC-MS/MS characterized redox status of each cysteine within circulating HMGB1 also is given for each time point.
b
Data are presented as mean ± SEM with six animals per group.
c
P < 0.01 versus 0 h.