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. Author manuscript; available in PMC: 2012 Apr 12.
Published in final edited form as: Nat Med. 2011 Jul 17;17(8):989–995. doi: 10.1038/nm.2422

Figure 4.

Figure 4

CFSE dilution data showing CD8+ T cells restricted by HLA-B*57 and HLA-B*27 resist Treg cell-mediated suppression in a GzmB dependent manner. (a) Percentage CFSEloCD8+ T cells after CFSE-labeled isolated CD8+ T cells were stimulated with their corresponding epitopes alone or together with Treg cells (at 1:0.25 ratio), and also in the presence or absence of a GzmB peptide inhibitor (z-AAD-CMK). Examples of flow data are shown in Supplementary Figure 3a. (b) Percentage CFSEloCD8+ T cells after electroporation with GZMB siRNA or nonhybridizing negative control (siControl) siRNA oligonucleotides and stimulation with their cognate epitopes alone or with Treg cells (at 1:0.25 ratio). Examples of flow data are shown in Supplementary Figure 3b.