Table 1.
ROS generation in NIH 3T3 cells expressing Nox1 and Nox1 plus h-catalase
| Cell line | Nox1 | h-catalase | Mean DCF fluorescence per cell | NBT reduced, nmol/20 min/106 cells |
|---|---|---|---|---|
| NEF2 | − | − | 9 ± 0.7 | 19.1 ± 0.5 |
| YA28 | + | − | 50 ± 5.2 | 28.7 ± 5.1 |
| YA28/Z4 | + | − | 41 ± 0.4 | 35.1 ± 2.9 |
| YA28/Z3 | + | − | 67 ± 1.0 | 76.2 ± 29 |
| ZC-1 | + | + | 25 ± 0.2 | 32.6 ± 3.2 |
| ZC-5 | + | + | 33 ± 0.5 | 30.5 ± 4.4 |
DCF fluorescence (a measure of H2O2) and
NBT reduction (a measure of O
) in the indicated cell
lines were measured as in Materials and Methods. The mean
and SE of three independent measurements are shown.