Table 1.
Summary of the Roche 454 and Illumina GAIIx data used for assembling P. fucata genome sequences
Used data |
Assembly |
|||||||
---|---|---|---|---|---|---|---|---|
Totalsequences (Gb) | Number of reads(million) | Average readlength | Estimatedcoverage | Contig |
Scaffold |
|||
Number | N50 (bp) | Number | N50 (bp) | |||||
454 | ||||||||
Shotgun | 15.2 | 44.5 | 341 | 16.3x | 1 085 563 | 1629 | 800 982 | 14 455 |
4 kb paired enda | 1.1 | 3.8 | 286 | |||||
10 kb paired enda | 2.4 | 7.8 | 309 | |||||
GAIIx | ||||||||
Shotgun | 3.9 | 76 | 50 | 26.7x | ||||
3 kb mate-pair | 10.9 | 216 | 50 | |||||
10 kb mate-pair | 11.9 | 238 | 50 |
aEach 454 raw reads were separated into ‘forward’ and ‘reverse’.