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The intracellular domain of HER4, s80HER4, is expressed from a tetracycline inducible promoter in HeLa cells. Induction of s80HER4 inhibits growth. Representative histograms of HeLa-pcDNA4 and HeLa-s80Her4 cells grown in serum-free medium ± tetracycline (48 h) and stained with propidium iodide and analyzed by flow cytometry. Percentage of cells in the G2/M phase of the cell cycle are shown, *P<0.002, Student’s unpaired t test.
