SDS/PAGE of ABD-T fractions from rat liver and ABD-T. The sample was
prepared as described in Materials and Methods,
subjected to HPLC as described in the legend of Fig. 2. The ABD-T peak
fraction was pooled, and then analyzed on a 10–20% Tris-Tricine SDS
gel with fluorescence detected on a UV transilluminator. Lane 1,
prestained protein standard. Lane 2, ABD-T fraction from rat liver.
Lane 3, ABD-F modified, purified rabbit zinc MT-2.