Figure 7.

fmi-1 is expressed in the nervous system, but not VD motorneurons. A, A Pfmi-1::GFP was used to determine the spatial expression of fmi-1. GFP was observed in a subset of neurons near the nerve ring (NR) along the ventral nerve cord (VNC) and in the lumbar ganglion (LG). B–E, To identify which motorneurons were expressing flamingo, we coexpressed Pfmi-1-gfp with either Pacr-2-rfp (B) or Punc-25-rfp (C–E). B, C, In larva and adults, the GFP-positive motorneurons also expressed Pacr-2-rfp, but not Punc-25::rfp. D, In contrast, none of the older larvae or adults coexpressed GFP and RFP when we used Punc-25-rfp. D–D″, In L1 larvae, there was limited coexpression of Punc-25-rfp and Pfmi-1-gfp in a subset of the DD motorneurons (arrows). The GFP intensity was much reduced relative to other FMI-1(+) cells. The remaining GFP-positive cells in the ventral cord are likely the DA motorneurons. E–E″, By the adult stage, DD1 (arrow) no longer expresses GFP. Scale bars, 10 μm.