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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Integr Biol (Camb). 2012 Feb 10;4(4):360–367. doi: 10.1039/c2ib00148a

Fig. 2. Single cell analysis of stem cells is enabled by bioengineered hydrogel microwell arrays.

Fig. 2

(A–D) Scheme of PDMS microwell replica generation. (E–G) Production of PEG microwell arrays. (H) Scheme of a hydrogel microwell array in a single well of a 24 well plate. (I) Protein functionalization. Vinyl sulfone is attached to protein through reaction with lysine residues. Functionalized protein can then be polymerized into the network. (J–M) Microcontacting printing and production of hydrogel microwell arrays. (J) Microcontact printing of protein onto PDMS stamp; (K) stamp with protein adsorbed on posts is placed on a glass slide with a drop of PEG precursor; (L) Hydrogel is polymerized at 37 °C for 1 h. (M) Stamp is removed leaving behind 24 bottom functionalized hydrogel arrays per slide.