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Effects of temperature on Tfn-induced SPR variation and real-time fluorescence measurements of Tfn uptake. (A) SPR measurements. Measurements were carried out as in Fig. 1. The half-life times (t1/2) of kinetics measured at 37°C and 30°C are 1.6 and 6.6 min, respectively. The process measured at 19°C is practically arrested. (B) Time-lapse fluorescence imaging measurements. Rhodamine Red holo-Tfn fluorescence accumulation at 37°C or 23°C was monitored as described in Fig. 2.
