Figure 4.

Kinetics of the Tfn-induced SPR reflectivity variation in human melanoma cells. Cells were grown on Au-coated ZnS prism. They were either untreated (circles), or subjected to cholesterol depletion by treatment with mβCD (rectangles), or cholesterol enriched by exposure to cholesterol complexed with mβCD (triangles). Cholesterol level was determined as described in Materials and Methods. (A) Raw data. Experimental data are indicated by the symbols. Solid lines are the fit to Eq. 3. (B) Normalized data. ΔR/ΔR_saturation. The dashed lines connect between the symbols. The inset describes the dependence of rate constants on cellular cholesterol levels.